This paper explores the application of traditional Chinese “Principle of Reason and Humanity” thought in cross-cultural communication and conflict resolution, aiming to provide insights into constructing universal moral and ethical principles. It first elucidates the profound wisdom of “Principle of Reason and Humanity” as the crystallization of Chinese civilization, highlighting its guiding significance for personal cultivation and interpersonal harmony. From a cross-cultural perspective, the paper demonstrates the unique role of “Principle of Reason and Humanity” in resolving civilizational conflicts and, through case studies, distills a systematic solution path of appealing to “affection,” persuading by “reason,” and regulating through “rule of law.” Building on this foundation, the paper discusses the enlightenment of the universal values embodied in “Principle of Reason and Humanity,” such as benevolence, justice, and good governance, for constructing universal ethical principles and envisions their innovative development in modern social contexts. This paper argues that in an era of increasingly frequent cross-cultural interactions, “Principle of Reason and Humanity” thought contributes to enhancing emotional identification, value consensus, and behavioral norms among different groups, providing essential ideological resources and practical paths for addressing global challenges and promoting the building of a community with a shared future for mankind.